Sciency Stuff-HIV

I read a very interesting article that had James Robinson as acoauthor. Oct 2005 Journal of Virology p12311-12320. Kinetic Rates ofAntibody binding correlate with neutralization sensitivity of variant simian immunodeficiency virus strains.

One thing that it pointed out was that temperature of runs was done at37 degrees C versus 25 degrees C. Since interactions occur in vivo at37 degrees C. They did studies at both temperatures and saw there wasa direct effect of temperature increase on rate constant. Increasingthe temperature to 37 degree C did not alter the KD, but did increasethe ka and kd of all three mAbs tested (3.8E, 3.11H, 1.10A)

How do you do global fits to a 1:1 langmuir model? The article impliedit designed the experiment so that there would be a good fit. I thinkI'm missing some important information.

I think it would be most interesting to determine if differences inHIV env binding proteins to mAbs( perhaps 2F5, 4E10, and otherneutralizing antibodies ) that are neutralizing and ones that are nonneutralizing using the biacore. It would be neat if we could find a correlary between neutralization sensitivity.